Biomiga SB-Green qPCR Master Mix, rox, a ready-to-use reaction cocktail that contains all components except primers and template for real-time quantitative PCR on systems that require rox input. The proprietary buffer and stabilizers have been optimized to deliver maximum PCR efficiency, sensitivity and robust fluorescent signal. The proprietary 2X qPCR Master Mix, Rox contains Hot Start Taq DNA polymerase, dNTPs, Mg2+, enhancer, stabilizer, and an optimal concentration of ROX for instruments that require ROX for a reference signal. Please consult our website and Instrument Compatibility section below for the correct product for your real-time PCR system.
2x qPCR Mix, SBGR, Rox
The system enables highly sensitive detection from few copies of DNA template, with a broad dynamic range that supports accurate quantification of target sequences.
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| Reactions |
100 Reactions, 500 Reactions |
|---|
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Сatalog number:
QP1318-03
One Step qRT-PCR 2x Mix 12.5 mL
Easy-to-use two tube system: Enzyme Mix (50X) and Reaction Mix (2X)
Broad range of RT reaction temperature (40-55oC)
Broad dynamic range of detection
Hot start PCR system
Fully optimized RT-qPCR buffer and robustness
High sensitivity, specificity, and reproducibility
Ideal for probe based COVID-19 viral RNA detection and quantitation
Mgpure Viral RNA Purification Kit
Purpose: This procedure describes the extraction of viral RNA from virus infected specimen for real-time RT-PCR detection.
Acceptable Specimens
Respiratory specimens including: nasopharyngeal or oropharyngeal aspirates or washes, nasopharyngeal or oropharyngeal swabs, broncheoalveolar lavage, tracheal aspirates, and sputum. o Swab specimens should be collected only on swabs with a synthetic tip with aluminum or plastic shafts. Swabs with calcium alginate or cotton tips with wooden shafts are not acceptable
Serum, plasma, or other body fluid.
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