PowerLoad™ is an optimized formulation of nonionic, Pluronic™ surfactant polyols for the solubilization of water-insoluble dyes and other materials in physiological media. These surfactants, for instance Pluronic™ F-127, have been used to help disperse acetoxymethyl (AM) esters of fluorescent ion indicators such as fluo-4, fura-2, indo-1, fluo-3, and SBFI; they appear to be required for loading of other dyes (e.g. SBFI-AM or PBFI-AM). The use of PowerLoad™ is optional with red shifted calcium indicators and other large molecular weight AM ester dyes, and may also be useful for dispersing other lipophilic probes. The concentration of Pluronic™ surfactants in PowerLoad™ is less than 0.2%. PowerLoad™ is effective in combination with water soluble Probenecid (P36400) to aid AM ester dye-loading and retention in cells that actively extrude the de-acetylated form through anion pumps. Together, these reagents allow for maximal loading of dyes with a minimum of effort in both imaging and high throughput screening (HTS) applications. Appropriate controls should be performed to make certain that PowerLoad™ is not altering the membrane properties of the cell. Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›
PowerLoad™ Concentrate, 100X
$169.00
Сatalog number:
P10020
Category: Laboratory Surfactants and Wetting Agents
Description
Additional information
Recommended Storage |
Store in refrigerator (2–8°C). |
---|---|
Quantity |
5 mL |
Reviews (0)
Be the first to review “PowerLoad™ Concentrate, 100X” Cancel reply
Shipping & Delivery
Related products
Сatalog number:
464252500
Tergitol(TM) 15-S-9
Сatalog number:
J64241.36
Sodium n-dodecyl sulfate, 97%, for electrophoresis, Thermo Scientific Chemicals
Сatalog number:
611950050
CHAPS
Сatalog number:
P6866
Pluronic™ F-127, 0.2 µm filtered (10% Solution in Water)
$134.00
Сatalog number:
L11435.30
Sorbitan monostearate, Thermo Scientific Chemicals
Сatalog number:
P6867
Pluronic™ F-127, low UV absorbance
$46.77
Сatalog number:
J61055.AP
Reviews
There are no reviews yet.