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What are ELISA Kits and Tests ?

What is ELISA Kit and Test ?

What are ELISA Kits and Tests ?

ELISA kits are ready-to-use immunoassay kits that contain all reagents required to perform the ELISA (Enzyme-Linked Immunosorbent Assay). ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying soluble substances such as peptides, proteins, antibodies, and hormones. Other names, such as enzyme immunoassay (EIA), are also used to describe the same technology.

The enzyme-linked immunosorbent assay (ELISA) is an immunological assay commonly used to measure antibodies, antigens, proteins and glycoproteins in biological samples. Some examples include: diagnosis of HIV infection, pregnancy tests, and measurement of cytokines or soluble receptors in cell supernatant or serum.
Enzyme-linked immunosorbent assay (ELISA) is a method of target antigen (or antibody) capture in samples using a specific antibody (or antigen), and of target molecule detection/quantitation using an enzyme reaction with its substrate.
If you need to detect or quantitate an analyte, then a sandwich or competitive ELISA can be utilized. However if you need to measure an immunological response, then a direct or indirect ELISA is most suitable for your needs.
Compared to ELISA, real-time PCR showed greater agreement among duplicate samples. ELISA was found to be less time consuming and easier to perform than real-time PCRELISA and real-time PCR showed 100% specificity during reference sample testing.
Test kits cost from $1.20 per test for ELISA to more than $30 for western blot.

6 thoughts on “What are ELISA Kits and Tests ?

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