One-Step RT-PCR is designed for the reverse transcription (RT) and polymerase chain reaction (PCR)
amplification of a specific target RNA from either total RNA or mRNA. This system uses a mixture of
MMLV Reverse Transcriptase and Hot start Taq DNA polymerase in an optimized reaction buffer, and
can detect RNA targets up to 4.0 kb. The amount of input total RNA can range from 0.01 pg to 2 μg.
Sufficient reagents are provided for 100 amplification reactions of 50 μl each.
One-Step RT-PCR Kit
Description
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| Preps |
100 preps, 25 preps, 5 preps |
|---|
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Mgpure Viral RNA Purification Kit
Purpose: This procedure describes the extraction of viral RNA from virus infected specimen for real-time RT-PCR detection.
Acceptable Specimens
Respiratory specimens including: nasopharyngeal or oropharyngeal aspirates or washes, nasopharyngeal or oropharyngeal swabs, broncheoalveolar lavage, tracheal aspirates, and sputum. o Swab specimens should be collected only on swabs with a synthetic tip with aluminum or plastic shafts. Swabs with calcium alginate or cotton tips with wooden shafts are not acceptable
Serum, plasma, or other body fluid.
Сatalog number:
QP1318-03
One Step qRT-PCR 2x Mix 12.5 mL
Easy-to-use two tube system: Enzyme Mix (50X) and Reaction Mix (2X)
Broad range of RT reaction temperature (40-55oC)
Broad dynamic range of detection
Hot start PCR system
Fully optimized RT-qPCR buffer and robustness
High sensitivity, specificity, and reproducibility
Ideal for probe based COVID-19 viral RNA detection and quantitation
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