One-Step RT-PCR is designed for the reverse transcription (RT) and polymerase chain reaction (PCR)
amplification of a specific target RNA from either total RNA or mRNA. This system uses a mixture of
MMLV Reverse Transcriptase and Hot start Taq DNA polymerase in an optimized reaction buffer, and
can detect RNA targets up to 4.0 kb. The amount of input total RNA can range from 0.01 pg to 2 μg.
Sufficient reagents are provided for 100 amplification reactions of 50 μl each.
One-Step RT-PCR Kit
Description
Additional information
| Preps |
100 preps, 25 preps, 5 preps |
|---|
Reviews (0)
Shipping & Delivery
Related products
Mgpure Viral RNA Purification Kit
Purpose: This procedure describes the extraction of viral RNA from virus infected specimen for real-time RT-PCR detection.
Acceptable Specimens
Respiratory specimens including: nasopharyngeal or oropharyngeal aspirates or washes, nasopharyngeal or oropharyngeal swabs, broncheoalveolar lavage, tracheal aspirates, and sputum. o Swab specimens should be collected only on swabs with a synthetic tip with aluminum or plastic shafts. Swabs with calcium alginate or cotton tips with wooden shafts are not acceptable
Serum, plasma, or other body fluid.
Сatalog number:
PK2020-02
Proteinase K 100 mL 25 mg/mL
Viral DNA/RNA Extraction from Respiratory Samples
The EZgeneTM Viral DNA/RNA mini kit provides an easy and reliable method for isolating total viral RNA from plasma, serum, nasopharyngeal or oropharyngeal aspirates or washes, nasopharyngeal or oropharyngeal swabs, broncheoalveolar lavage, tracheal aspirates, and sputum. This procedure has been tested for isolating nucleic acids from COVID-19, Hepatitis A, Hepatitis C and HIV. The isolated RNA can be used for PCR, qRT-PCR and other downstream applications.
Reviews
There are no reviews yet.