The Autopure 32 MgPureTM Viral RNA purification kit provides an easy and reliable method for isolating total viral RNA from plasma, serum, nasopharyngeal or oropharyngeal aspirates or washes, nasopharyngeal or oropharyngeal swabs, broncheoalveolar lavage, tracheal aspirates, and sputum. This procedure has been tested for isolating nucleic acids from COVID-19, Hepatitis A, Hepatitis C and HIV. The isolated RNA can be used for PCR, qRT-PCR and other downstream applications. This protocol is validated on Allsheng auto pure 96 and KingFisher plex and can be adapted to major automation platforms such as Biomek FX, Biomek 3000, Hamilton Microlab STAR, Hamilton MagEx STARle and many others.
MgPure Viral RNA, pre-plated for AllSheng AutoPure 32
Description
Additional information
| Samples |
100×16 samples, 10×16 samples, 40×16 samples |
|---|
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Сatalog number:
AS4800
Allsheng AutoPure 4800
Сatalog number:
QP1318-03
One Step qRT-PCR 2x Mix 12.5 mL
Easy-to-use two tube system: Enzyme Mix (50X) and Reaction Mix (2X)
Broad range of RT reaction temperature (40-55oC)
Broad dynamic range of detection
Hot start PCR system
Fully optimized RT-qPCR buffer and robustness
High sensitivity, specificity, and reproducibility
Ideal for probe based COVID-19 viral RNA detection and quantitation
Mgpure Viral RNA Purification Kit
Purpose: This procedure describes the extraction of viral RNA from virus infected specimen for real-time RT-PCR detection.
Acceptable Specimens
Respiratory specimens including: nasopharyngeal or oropharyngeal aspirates or washes, nasopharyngeal or oropharyngeal swabs, broncheoalveolar lavage, tracheal aspirates, and sputum. o Swab specimens should be collected only on swabs with a synthetic tip with aluminum or plastic shafts. Swabs with calcium alginate or cotton tips with wooden shafts are not acceptable
Serum, plasma, or other body fluid.
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