RNA Purification Columns

Purpose: This procedure describes the extraction of viral RNA from virus infected specimen for real-time RT-PCR detection. Acceptable Specimens Respiratory specimens including: nasopharyngeal or oropharyngeal aspirates or washes, nasopharyngeal or oropharyngeal swabs, broncheoalveolar lavage, tracheal aspirates, and sputum. o Swab specimens should be collected only on swabs with a synthetic tip with aluminum or plastic shafts. Swabs with calcium alginate or cotton tips with wooden shafts are not acceptable Serum, plasma, or other body fluid.