Regeneration Buffer， 2 x Elution Buffer， 10 x Wash Buffer， 50 mL Collection Tube， Desalting Tube， Press-On Cap，Maxi Columns
Introduction
The ViraTrapTM retrovirus mini purification kit is designed for fast and efficient purification of recombinant retroviruses from retrovirus-transfected cell culture supernatant. Up to 3 x1012 viral particles can be purified from cell culture media of 1 to 2 T75 flasks.
Traditionally the recombinant retrovirus is purified by ultracentrifugation using CsCl to separate the virus particles from cellular proteins and media components. The CsCl ultracentrifugation procedure is time consuming and limited to the amount of cell lysate to be processed.
Each column can be regenerated for purifying the same retrovirus. For optimized viral binding and recovery, each column can be regenerated only once.
Storage and Stability
All components are guaranteed for at least 12 months from the date of purchase when stored as follows: Mini column and desalting tube should be stored at 4 °C, and all other materials at RT (22-25°C).
Before Starting
Familiar with each step by reading this menu and prepare all materials for the procedure.
Safety Considerations
The retrovirus infected cell media and the purified virus can be potential bio-hazardous material and can be infectious to human and animals. All protocols MUST be performed under at least Bio-Safety level 2 working condition.
Materials Required but Not Supplied
- ddH2O
- PBS
- 0.45 μm filters
- Rack holder for columns
Kit Contents
| Catalog# | V1172-00 | V1172-01 | V1172-02 | Notes |
| Preps | 2 | 10 | 20 | |
| Press-On Cap | 1 | 5 | 10 | Store at RT |
| Columns | 1 | 5 | 10 | Can be used twice(Store at 4°C) |
| Desalting Tube* | Not included | 1 | 1 | Can be regenarated(Store at 4°C) |
| 15mL Collection Tube | 2 | 10 | 20 | Store at RT |
| 10 x Wash Buffer | 6 mL | 30 mL | 60 mL | Store at RT |
| 2 x Elution Buffer | 6 mL | 30 mL | 60 mL | Store at RT |
| Regeneration Buffer | 6 mL | 30 mL | 60 mL | Store at RT |
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